Abstract: Objective To verify the bactericidal effect of 5 kinds of disinfectants on 4 kinds of bacteria which must be excluded in SPF experiment animals, this study was performed. Method D / E neutralizing broth was used as neutralizing agent to investigated its neutralizing effect on 5 disinfectants. The bactericidal effects of the 5 disinfectants on Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhimurium were investigated by suspension quantitative bactericidal test. Result D / E neutralization broth had good neutralization effect on bromogeramine solution, Jianzhisu disinfectant, Didecyl dimethyl ammonium bromide solution, BIOCID-30 and 75% alcohol solution. The bromogeramine solution, Jianzhisu disinfectant, BIOCID-30 and 75% alcohol solution had good killing effect after 1. 5 min respectively with four kinds of bacteria. The 600 times diluted Didecyl dimethyl ammonium bromide solution could effectively kill Staphylococcus aureus,  Klebsiella pneumoniae and Salmonella typhimurium in 5 - 10 min, while its bactericidal effect on Pseudomonas aeruginosa was not ideal. The 200 times diluted Didecyl dimethyl ammonium bromide solution could kill Pseudomonas aeruginosa effectively for 1. 5 min. Conclusion The bromogeramine solution, Jianzhisu disinfectant, BIOCID-30 and 75% alcohol solution could produce good bactericidal effect on four kinds of bacteria within a short time. A higher concentration of Didecyl dimethyl ammonium bromide solution could effectively kill Pseudomonas aeruginosa.

Key words: disinfectants, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, bactericidal effect

摘要： 目的 验证 5 种消毒剂对 4 种 SPF 级实验动物必须排除的细菌的杀菌效果。 方法 以 D / E 中和肉汤为中和剂,检测其对 5 种消毒剂的中和作用;通过悬液定量杀菌实验,测试 5 种消毒剂对金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌和鼠伤寒沙门氏菌的杀菌效果。 结果 D / E 中和肉汤对于新洁尔灭、健之素消毒液、百毒杀、百胜-30 和 75%乙醇均有较好的中和作用。 新洁尔灭、健之素消毒液、百胜-30 和 75%乙醇 4 种消毒剂分别与 4 种细菌作用,1. 5 min 后杀灭效果良好。 1 ∶ 600 倍稀释的百毒杀消毒液有效杀灭金黄色葡萄球菌、肺炎克雷伯菌、沙门氏菌需要 5 ~ 10 min,但它对铜绿假单胞菌杀菌效果不理想。 1 ∶ 200 倍稀释的百毒杀消毒液作用 1. 5 min,可以有效杀灭铜绿假单胞菌。 结论 新洁尔灭、健之素消毒液、百胜-30 和 75%乙醇在较短时间内即可对 4 种细菌产生良好的消毒效果。 百毒杀有效杀灭铜绿假单胞菌需要较高浓度。

关键词: 消毒剂, 金黄色葡萄球菌, 铜绿假单胞菌, 肺炎克雷伯菌, 沙门氏菌, 杀菌效果